Recently, the team of researchers from the Dalian Institute of Chemical Physics of the Chinese Academy of Sciences, Qin Jianhua, made new progress in the cultivation of human islet organs using organ chip technology. The results were published in the lab chip field Lab on a chip and were selected as cover articles.
Organoids are multicellular three-dimensional structures formed by self-assembly of stem cells in vitro, capable of mimicking specific structures and functions of the source organs, providing a new model for developmental biology, regenerative medicine, and disease research. It is considered to be a major technological breakthrough in recent years.
Diabetes seriously affects people's life and quality of life. The main pathological changes are progressive impaired islet function and can lead to various complications of the body. The construction of human islet organs in vitro is of great significance for diabetes replacement therapy, disease mechanism research and drug development.
Using human induced pluripotent stem cells (hiPSC) as the source, the researchers combined the principle of cutting-edge organ chip technology with the principle of stem cell self-assembly, and realized the endodermal directed differentiation of human pluripotent stem cells on the multi-layer array design of the perfusion chip. 3D dynamic culture and islet tissue formation; in vitro development of islet development, the islet organs with near physiological structure and functional characteristics were obtained. After identification, the islet-like organs cultivated on the chip contain various types such as α and β cells, and have good insulin secretion and sugar-stimulated response functions. In addition, the study also found that mechanical fluid stimulation in the culture environment is conducive to promoting the long-term maintenance of organ maturity and function, revealing the importance of mechanical factors in the tissue microenvironment for the construction of functional organs.
This work utilizes the collaborative strategy of bioengineering and development to realize the cultivation of organoids-on-chips on the chip, and further develops 3D classes that are more physiologically compliant through the multi-functional integration of the chip and accurate simulation of the tissue microenvironment. The organ model provides a new idea and platform for life science research and tissue organ manufacturing.
In the past two years, Qin Jianhua's research team has integrated the characteristics of multidisciplinary cross-extension, and has taken the lead in integrating organ chips and organ-like techniques to realize the construction of various functional organs such as brain, liver and islets on the chip, and used for tissue development and disease. Research on simulation and early life exposure has attracted the attention of international peers. (Reporter Liu Wansheng correspondent Tao Tingting)
Related links: DOI: 10.1039/C8LC01298A